Genome-wide analysis of uncapped mRNAs under heat stress in Arabidopsis

Abstract

Recently, we have showed that Tudor Staphylococcal Nuclease (TSN or Tudor-SN) proteins (TSN1 and TSN2) are localized in cytoplasmic messenger ribonucleoprotein (mRNP) complexes called stress granules (SG) and processing bodies (PB) under heat stress in Arabidopsis. One of the primary functions of these mRNP complexes is mRNA decay, which generates uncapped mRNAs by the action of endonucleases and decapping enzymes (Thomas et al., 2011) [1]. In order to figure out whether TSN proteins could be implicated in mRNA decay, we isolated uncapped and total mRNAs of Wild type (WT; Col and Ler) and TSN double knock-out (tsn1tsn2) seedlings grown under heat stress (39. °C for 40. min) and control (23. °C) conditions. Here, we provide the experimental procedure to reproduce the results (NCBI GEO accession number GSE63522) published by Gutierrez-Beltran et al. (2015) in The Plant Cell [2].

Keywords

Arabidopsis; Degradome; MRNA decay; Stress; Stress Granules

Published in

Genomics Data
2015, Volume: 5, pages: 7-8 Publisher: Elsevier Inc

SLU Authors

• Gutierrez, Emilio

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics


Publication identifier

DOI: https://doi.org/10.1016/j.gdata.2015.04.020

Permanent link to this page (URI)

https://res.slu.se/id/publ/132080