Abstract

A cell mediated immune (CMI) response was measured in vitro to heat-killed and to paraformaldehyde fixed Renibacterium salmoninarum (Rs) in rainbow trout (Oncorhynchus mykiss) experimentally challenged with live Rs. The mitogenic response to the T lymphocyte mitogen Concanavalin A (Con A) was reduced during samplings 4 to 6 weeks after immersion, but no effect of the response to the B lymphocyte mitogen lipopolysaccharide (LPS) was detected. The subpopulation of lymphocytes, detected by the monoclonal antibody 1C2, was decreased from the 4th week to the 5th week of infection, and remained at the decreased level up to 10 weeks post immersion. The proportion of Immunoglobulin (Ig) bearing lymphocytes was not affected during the Rs infection period. The humoral antibody level to heat-stable Rs-antigens was increased up to 10 weeks after immersion but after 27 weeks was reduced to a level similar to that of the non-challenged fish. An anamnestic response was demonstrated in challenged fish, as intraperitoneal injection of heat-treated Rs bacteria into Rs challenged fish elicited a stronger humoral antibody response compared with injection into non-challenged fish. (C) 2002 Elsevier Science Ltd. All rights reserved

Keywords

bacterial kidney disease; Renibacterium salmoninaru; experimental challenge; lymphocytes; lymphocyte stumulation; cell mediated immunity

Published in

Fish and Shellfish Immunology
2003, Volume: 14, number: 4, pages: 347-362
Publisher: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD

SLU Authors

• Johannisson, Anders

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Norrgren, Leif

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Environmental Sciences related to Agriculture and Land-use


Publication identifier

DOI: https://doi.org/10.1006/fsim.2002.0442

Permanent link to this page (URI)

https://res.slu.se/id/publ/1322