Molecular marker techniques in Musa genomic research

Abstract

Banana is one of the most important subtropical crops. Its genetic system is relatively unknown and complicated by inter- and intraspecific hybridization, heterozygosity, and polyploidy. Thus molecular markers have been widely used among Musa germplasm to detect genetic variation and relationships, duplicate accessions identification, monitoring genetic stability, analyzing irradiated genotypes and identification of trait markers for use in breeding programs. Various markers based morphology, protein, and DNA are in existence. Among them the most widely used markers suitable for marker assisted selection (MAS) are RFLP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLP), and simple sequence repeat (SSR). This chapter compares the efficiency of available markers, its application and development for Musa genome analysis. High-throughput technologies based on single nucleotide polymorphisms (SNPs) or small scale indels are efficient alternatives with desired dosage sensitivity and the ability to distinguish haplotypes in polyploid crops. However, the choice of markers differ and depends on the labor required for their detection, possibility of revealing single or multiple loci, dominant or co-dominant nature, and costs. Recent developments reported in various crops like high resolution DNA melting (HRM) analysis has great potential for cultivar identification, candidate gene identification, mapping (genomic and association) studies. Thus the identified genomic regions in banana with these approaches has the potential to dramatically enhance the pace and efficiency of genetic improvement in Musa.

Keywords

Genetic improvement; Molecular marker systems; Musa

Published in

Title: Genetics, Genomics, and Breeding of Bananas
Publisher: CRC Press

SLU Authors

• Ortiz Rios, Rodomiro Octavio

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics and Genomics
Biochemistry and Molecular Biology

Publication identifier

• DOI: https://doi.org/10.1201/b11776
• ISBN: 978-1-5780-8788-4
• eISBN: 978-1-4665-0516-2

Permanent link to this page (URI)

https://res.slu.se/id/publ/132845