Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Abstract
The objectives of this study were to evaluate the effect of a short, cooled storage before cryopreservation on sperm progressive motility (PM) and compare the effect of different centrifugation methods on post-thaw PM of stored samples. Semen was diluted in chilling extender and aliquoted in 6 protocols: i) Standard centrifugation (SC) followed by freezing; ii) Single Layer Centrifugation (SLC) followed by freezing; iii) Storage for 8 h/5 degrees C before SC; iv) Storage for 8 h/5 degrees C before SLC; v) Storage for 8 h/15 degrees C before SC; and vi) Storage for 8 h/15 degrees C before SLC. PM was assessed before centrifugation, after centrifugation, and post-thawing. Stallions were classified as "good freezers" (GF) or "bad freezers" (BF). The PM in samples immediately frozen was greater than in the stored ones (71.98 +/- 14.2, 52.91 +/- 17.8, 53.93 +/- 18.9 for no storage, 5 degrees C storage and 15 degrees C storage, respectively) (P
Keywords
Cryopreservation; Extender; Single layer centrifugation; Sperm
Published in
Journal of Equine Veterinary Science
2024, volume: 135, article number: 105046
SLU Authors
UKÄ Subject classification
Clinical Science
Publication identifier
- DOI: https://doi.org/10.1016/j.jevs2024.105046
Permanent link to this page (URI)
https://res.slu.se/id/publ/139316