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Research article2006Peer reviewed

Functional division of intrinsic neurons in the mushroom bodies of male Spodoptera littoralis revealed by antibodies against aspartate, taurine, FMRF-amide, Mas-allatotropin and DC0

Sjoholm M, Sinakevitch I, Strausfeld NJ, Ignell R, Hansson BS

Abstract

The aim of this study was to further reveal the organization of Kenyon cells in the mushroom body calyx and lobes of the male moth Spodoptera littoralis, by using immunocytochemical labeling. Subdivisions of the mushroom bodies were identified employing antisera raised against the amino acids taurine and aspartate, the neuropeptides FMRF-amide and Mas-allatotropin, and against the protein kinase A catalytic subunit DC0. These antisera have previously been shown to label subsets of Kenyon cells in other species. The present results show that the organization of the mushroom body lobes into discrete divisions, described from standard neuroanatomical methods, is confirmed by immunocytology and shown to be further elaborated. Anti-taurine labels the accessory Y-tract, the gamma division of the lobes, and a thin subdivision of the most posterior component of the lobes. Aspartate antiserum labels the entire mushroom body. FMRF-amide-like immunolabeling is pronounced in the gamma division and in the anterior perimeter of the alpha/beta and alpha'/beta' divisions. Mas-allatotropin-like immumolabeling shows the opposite of FMRF-amide-like and taurine-like immunolabeling: the gamma division and the accessory Y-system is immunonegative whereas strong labeling is seen in both the alpha/beta and alpha'/beta' divisions. The present results agree with findings from other insects that mushroom bodies are anatomically divided into discrete parallel units. Functional and developmental implications of this organization are discussed. (c) 2006 Elsevier Ltd. All rights reserved

Published in

Arthropod Structure and Development
2006, Volume: 35, number: 3, pages: 153-168
Publisher: ELSEVIER SCI LTD

      SLU Authors

    • Ignell, Rickard

      • Department of Crop Science, Swedish University of Agricultural Sciences

      • Hansson, Bill

        • Department of Crop Science, Swedish University of Agricultural Sciences

      Publication identifier

      DOI: https://doi.org/10.1016/j.asd.2006.03.001

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/14065