Carlson-Nilsson, Ulrika
- Nordic Genetic Resource Center (NordGen)
Abstract
Background Plant mutagenesis creates novel alleles, thereby increasing genetic and phenotypic diversity. The availability of the faba bean (Vicia faba L.) reference genome and a growing set of additional genomic resources has increased the scientific and practical value of mutant collections. We aimed to genotype and morphologically phenotype a historical faba bean mutant collection developed and characterized by Jan Sjodin (1934-2023) over half a century ago in order to increase its value to researchers. The collection was genotyped using high-throughput single-primer enrichment technology (SPET) assays. Results We used 11,073 informative single nucleotide polymorphism (SNP) markers spanning the faba bean genome to genotype 52 mutant lines along with the background line, cv. Primus. A range of flower, seed, leaf, and stipule mutations were observed. The analysis of population structure revealed a shallow structure with no major subpopulations. Principal component and cluster analyses revealed, to a minor extent, that the mutants clustered by their phenotype. Conclusions The mutants' phenotypic variation and shallow structure indicate that the Sjodin faba bean collection has the potential to play a significant role in faba bean breeding and in genetic and functional studies.
Keywords
Vicia faba; Mutant genetic resources; Genetic diversity; SPET genotyping
Published in
HEREDITAS
2024, volume: 161, number: 1, article number: 37
Publisher: BMC
SLU Authors
UKÄ Subject classification
Genetics and Breeding in Agricultural Sciences
Publication identifier
- DOI: https://doi.org/10.1186/s41065-024-00339-7
Permanent link to this page (URI)
https://res.slu.se/id/publ/142339