Skip to main content
SLU publication database (SLUpub)

Research article2006Peer reviewed

Biochemical characterization and identification of ice-nucleation-active (INA) willow pathogens by means of BIOLOG (R) MicroPlate, INA gene primers and PCR-based 16S rRNA-gene analyses

Nejad, P; Ramstedt, M; Granhall, U; Roos, S; McIvor, I

Abstract

Detection and identification of ice-nucleation active (INA) bacteria, was carried out in several independent investigations from diseased willow plants in different regions in Sweden and Estonia. Many of these bacteria, alone or together, cause serious bacterial disease problems in willow (Salix spp.) plants in combination with frost leading to dieback in plantations for energy forestry purposes. Methods used for identification were BIOLOG (R) MicroPlates, biochemical tests including growth in different media and pathogenic tests, designing and using selective INA primers, and 16S rRNA gene analyses. The taxonomic tools, especially phylogenetic analysis derived from 16S rRNA gene sequences, clearly distinguished many bacteria. The identified strains from willows (20 clones) belonged to at least eight different genera and 12 species showing variable levels of aggressiveness and ice-nucleation activity under laboratory and greenhouse conditions. Diseased willows were found associated with the presence of Agrobacterium tumefaciens, Bacillus spp., Clavibacter spp., Erwinia rhapontici, Frigoribacterium. faeni, Pseudomonas brenneri, P. fluorescens, P. frederiksbergensis, P. graminis, P. syringae, P. veronii, Sphingobacterium/ Pedobacter, Sphingomonas/non-fluorescent P. fluorescens (different biotypes), Xanthomonas campestris and related species

Published in

Journal of Plant Diseases and Protection
2006, volume: 113, number: 3, pages: 97-106
Publisher: EUGEN ULMER GMBH CO

SLU Authors

UKÄ Subject classification

Forest Science
Bioenergy

Publication identifier

  • DOI: https://doi.org/10.1007/BF03356165

Permanent link to this page (URI)

https://res.slu.se/id/publ/14250