Abstract
Tocols are natural antioxidants that occur in grains that may benefit human and animal health. Therefore, it is important to accurately measure their concentrations in foods and feeds and to determine how genetics and growing environment can influence their levels. The first objective was to evaluate saponification versus direct extraction for the analysis of tocols in oat (Avena sativa L.). The second was to determine the effects of growing environment, hulled versus hulless phenotype, and genetic background on tocol concentration, and to see whether tocol and lipid concentrations were associated. For the first objective, oat grain samples from two locations were either extracted by saponification or directly with methanol, and extracts were analyzed by HPLC. The saponification method increased yield by approximate to 25% and was less time-consuming, so it was adopted for the second objective. For the second objective, oat genotypes were developed by crossing high-oil parents from Iowa State University with hulled and hulless cultivars adapted to arid Western environments. These were grown at Aberdeen and Tetonia, ID, and the tocols and lipid concentrations were analyzed at Madison, WI. There were significant effects of growing environment, genotype, and the presence or absence of hulls on tocol concentrations. Tocol and lipid concentrations were not correlated. Progeny of crosses involving the genotype IA91098-2 had tocol concentrations that exceeded both parents.
Keywords
Analytic method; antioxidant; cultivation influence; genotypic influence; kernel content; oil; oat; tocopherol; tocotrienol
Published in
Cereal Chemistry
2007, volume: 84, number: 1, pages: 56-60
Publisher: AMER ASSOC CEREAL CHEMISTS
UKÄ Subject classification
Food Science
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1094/CCHEM-84-1-0056
Permanent link to this page (URI)
https://res.slu.se/id/publ/14361