Uggla, Arvid
- Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences
Abstract
Two Toxoplasma gondii antigen preparations consisting of extracted T. gondii RH strain tachyzoite cell membrane proteins arranged into submicroscopical particles (iscoms) were prepared from organisms propagated in mouse peritoneal cavities (A) and cell culture (B), respectively, and the preparations used as vaccine at two doses of 10 μg and 5 μg protein with 42 days apart. Eight days after the second vaccination, high dye test titres as well as significant delayed-type hypersensitivity reactions (footpad assay) were recorded in mice vaccinated with both preparations used, and the highest dye test titres (> 1:4,096) seen in mice vaccinated with the iscom A preparation. After intraperitoneal challenge on the same day with 104 or 105 T. gondii C56 strain tachyzoites, no control mice survived longer than 18 or 10 days, respectively, whereas 40 days after challenge mice vaccinated with the iscom A preparation had accumulated mortality rates of 11% and 37.5%, respectively, and iscom B mice 17% at both challenge doses employed.
Published in
Journal of veterinary medicine. Series B
1988, volume: 35, number: 4, pages: 311-314
SLU Authors
UKÄ Subject classification
Clinical Science
Publication identifier
- DOI: https://doi.org/10.1111/j.1439-0450.1988.tb00501.x
Permanent link to this page (URI)
https://res.slu.se/id/publ/145184