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Research article - Peer-reviewed, 2003

Preparation of a calibrant as certified reference material for determination of the Fusarium mycotoxin zearalenone

Josephs RD, Krska R, MacDonald S, Wilson P, Pettersson H


In the field of mycotoxin analysis, substantial problems shown by high between-laboratory standard deviations and noncomparable and nontraceable results have been caused by the lack of proper calibrants for external calibration. During a large-scale Standard Measurement and Testing project of the European Commission (EC) dealing with preparation and certification of reference materials for determination of the mycotoxin zearalenone (ZON) in maize, a ZON calibrant in acetonitrile was prepared and checked for purity, homogeneity, and stability. Before certification, on the basis of preparation, the calibrant was checked in a mini-interlaboratory study by UV spectrophotometric determination. The molar absorptivities of ZON in acetonitrile at 236, 274, and 314 nm were established, and as a main result, a common reference wavelength of 274 nm with molar absorptivity of 12 623 +/- 111 L/ can be recommended for ZON in acetonitrile. A concentration and expanded uncertainty of the ZON calibrant of 9.95 +/- 0.08 mug/mL was calculated as a preliminary value before final evaluation through the certification panel of the EC


zearalenone; mycotoxin

Published in

2003, volume: 86, number: 1, pages: 50-60

Authors' information

Pettersson, Hans
Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management
Wilson, Pete
Josephs, Ralf D
Krska, Rudolf
MacDonald, Susan

UKÄ Subject classification

Food Science
Veterinary Science
Animal and Dairy Science

URI (permanent link to this page)