Research article - Peer-reviewed, 2003
Preparation of a calibrant as certified reference material for determination of the Fusarium mycotoxin zearalenone
Josephs RD, Krska R, MacDonald S, Wilson P, Pettersson HAbstract
In the field of mycotoxin analysis, substantial problems shown by high between-laboratory standard deviations and noncomparable and nontraceable results have been caused by the lack of proper calibrants for external calibration. During a large-scale Standard Measurement and Testing project of the European Commission (EC) dealing with preparation and certification of reference materials for determination of the mycotoxin zearalenone (ZON) in maize, a ZON calibrant in acetonitrile was prepared and checked for purity, homogeneity, and stability. Before certification, on the basis of preparation, the calibrant was checked in a mini-interlaboratory study by UV spectrophotometric determination. The molar absorptivities of ZON in acetonitrile at 236, 274, and 314 nm were established, and as a main result, a common reference wavelength of 274 nm with molar absorptivity of 12 623 +/- 111 L/mol.cm can be recommended for ZON in acetonitrile. A concentration and expanded uncertainty of the ZON calibrant of 9.95 +/- 0.08 mug/mL was calculated as a preliminary value before final evaluation through the certification panel of the ECKeywords
zearalenone; mycotoxinPublished in
Journal of AOAC INTERNATIONAL2003, volume: 86, number: 1, pages: 50-60
Publisher: AOAC INTERNATIONAL
Authors' information
Pettersson, Hans
Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management
Wilson, Pete
Josephs, Ralf D
Krska, Rudolf
MacDonald, Susan
UKÄ Subject classification
Food Science
Veterinary Science
Animal and Dairy Science
URI (permanent link to this page)
https://res.slu.se/id/publ/1452