Research article - Peer-reviewed, 2007
Community structure of actively growing bacterial populations in plant pathogen suppressive soil
Hjort K, Lembke A, Speksnijder A, Smalla K, Jansson JKAbstract
The bacterial community in soil was screened by using various molecular approaches for bacterial populations that were activated upon addition of different supplements. Plasmodiophora brassicae spores, chitin, sodium acetate, and cabbage plants were added to activate specific bacterial populations as an aid in screening for novel antagonists to plant pathogens. DNA from growing bacteria was specifically extracted from the soil by bromodeoxyuridine immunocapture. The captured DNA was fingerprinted by terminal restriction fragment length polymorphism (T-RFLP). The composition of the dominant bacterial community was also analyzed directly by T-RFLP and by denaturing gradient gel electrophoresis (DGGE). After chitin addition to the soil, some bacterial populations increased dramatically and became dominant both in the total and in the actively growing community. Some of the emerging bands on DGGE gels from chitin-amended soil were sequenced and found to be similar to known chitin-degrading genera such as Oerskovia, Kitasatospora, and Streptomyces species. Some of these sequences could be matched to specific terminal restriction fragments on the T-RFLP output. After addition of Plasmodiophora spores, an increase in specific Pseudomonads could be observed with Pseudomonas-specific primers for DGGE. These results demonstrate the utility of microbiomics, or a combination of molecular approaches, for investigating the composition of complex microbial communities in soilPublished in
Microbial Ecology2007, volume: 53, number: 3, pages: 399-413
Publisher: SPRINGER
Authors' information
Hjort, Karin
Swedish University of Agricultural Sciences, Department of Microbiology
Jansson, Janet
Swedish University of Agricultural Sciences, Department of Microbiology
Lembke, Antje
Speksnijder, Arjen
Smalla, Kornelia
UKÄ Subject classification
Agricultural Science
Publication Identifiers
DOI: https://doi.org/10.1007/s00248-006-9120-2
URI (permanent link to this page)
https://res.slu.se/id/publ/14620