Research article - Peer-reviewed, 2007
Near-infrared spectroscopy for estimation of ergosterol content in barley: A comparison between reflectance and transmittance techniques
Borjesson T, Stenberg B, Schnurer JAbstract
The fungal-specific lipid ergosterol correlates with fungal biomass and often also with the degree of mycotoxin contamination of cereals. We compared the ability of a near-infrared reflectance (NIR) instrument with a broad wavelength range (400-2500 nm) and a near-infrared transmittance (NIT) instrument with a narrower wavelength range (850-1050 nm) to predict the ergosterol content of naturally infected barley samples. The two instruments were equally good at predicting ergosterol content in Swedish samples (r(2) = 0.81 and 0.83 for NIT and NIR, respectively). The NIT instrument was then used for samples from three countries (Sweden, Ireland, UK). This model had about the same root mean-squared error (approximate to 5 mg of ergosterol/kg, db, of grain) as the dataset with only Swedish samples, although the r(2) value was lower (0.58). The investigation has shown that it is possible to predict ergosterol content in whole barley samples using NIR or NIT instrumentation, and acceptable models can be obtained using different barley cultivars and samples from different countries and harvest years. This should make it possible to routinely predict the fungal biomass at grain terminalsPublished in
Cereal Chemistry2007, volume: 84, number: 3, pages: 231-236
Publisher: AMER ASSOC CEREAL CHEMISTS
Authors' information
Swedish University of Agricultural Sciences, Department of Soil Sciences
Swedish University of Agricultural Sciences, Department of Microbiology
Börjesson, Thomas
UKÄ Subject classification
Agricultural Science
Food Science
Publication Identifiers
DOI: https://doi.org/10.1094/CCHEM-84-3-0231
URI (permanent link to this page)
https://res.slu.se/id/publ/14640