Adomas, Aleksandra
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
Research article2007Peer reviewed
Transcript profiling of a conifer pathosystem: response of Pinus sylvestris root tissues to pathogen (Heterobasidion annosum) invasion
Adomas, Aleksandra; Heller, Gregory; Li, Guosheng; Olson, Åke; Stenlid, Jan; Finlay, Roger; Asiegbu, Frederick
Abstract
The mechanisms underlying defence reactions to a pathogen attack, though well studied in crop plants, are poorly understood in conifers. To analyze changes in gene tran- script abundance in Pinus sylvestris L. root tissues infected by Heterobasidion annosum (Fr.) Bref. s.l., a cDNA microarray containing2109ESTsfromP.taedaL.wasused.Mixedmodel statistical analysis identified 179 expressed sequence tags dif- ferentially expressed at 1, 5 or 15 days post inoculation. In gen- eral, the total number of genes differentially expressed during the infection increased over time. The most abundant group of genes up-regulated upon infection coded for enzymes involved in metabolism ( phenylpropanoid pathway) and defence-re- lated proteins with antimicrobial properties. A class III peroxidase responsible for lignin biosynthesis and cell wall thickening had increased transcript abundance at all measure- ment times. Real-time RT-PCR verified the microarray results with high reproducibility. The similarity of the expression pro- filing pattern observed in this pathosystem to those docu- mented in crop pathology suggests that angiosperms and gym- nosperms use similar genetic programs in responding to inva- sive growth by microbial pathogens.
Keywords
antimicrobial peptide; microarray; peroxidse; phenylopropanoid pathway; pine; plant defence
Published in
Tree Physiology
2007, volume: 27, number: 10, pages: 1441-1458
SLU Authors
Heller, Gregory
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
Li, Guosheng
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
Olson, Åke
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
Stenlid, Jan
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
Finlay, Roger
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
Asiegbu, Frederick
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
- Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Genetics and Genomics
Bioinformatics and Computational Biology (Methods development to be 10203)
Microbiology
Publication identifier
- DOI: https://doi.org/10.1093/treephys/27.10.1441
Permanent link to this page (URI)
https://res.slu.se/id/publ/14684