Two hydrophobin genes from the conifer pathogen Heterobasidion annosum are expressed in aerial hyphae

Abstract

Two hydrophobin genes (HAH1 and HAH2) have been identified in a Helerobasidion annosum infection-stage cDNA-library. Comparisons of their nucleotide and amino acid sequences show similarity to the coh1 hydrophobin from Coprinopsis cinerea and the sc3 hydrophobin from Schizophyllum commune. Both HAH1 and HAH2 display the amino acid consensus pattern of class I hydrophobins, including the spacing of eight conserved cysteine residues. Real-time quantitative RT-PCR showed high expression of both genes in aerial hyphae but low expression in submerged hyphae and during in vitro infection of pine seedlings. Segregation analysis of HAH1 and HAH2 in a defined cross of Helerobasidion annosum localised HAM to linkage group 3 but did not positioned HAH2 in the genetic linkage map. Sequence characteristics and expression patterns of HAH1 and HAH2 suggest a role in aerial growth of mycelia, but not during pathogenesis

Keywords

conifer root rot; forest pathology; gene expression; gene structure

Published in

Mycologia
2007, Volume: 99, number: 2, pages: 227-231 Publisher: ALLEN PRESS INC

SLU Authors

• Karlsson, Magnus

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Stenlid, Jan

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Olson, Åke

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Environmental Sciences related to Agriculture and Land-use


Publication identifier

DOI: https://doi.org/10.3852/mycologia.99.2.227

Permanent link to this page (URI)

https://res.slu.se/id/publ/14761