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Research article - Peer-reviewed, 2003

Purity assessment of crystalline zearalenone

Krska R, Welzig E, Josephs RD, Kandler W, Pettersson H, MacDonald S, Charlton A, Brereton P, Hametner C, Berner D, Zollner P


Commercially available solid zearalenone (ZON) to be used as a certified liquid calibrant (BCR-699) in a project funded by the European Commission within the Standard Measurement and Testing program was characterized and its purity determined. The degree of purity of the ZON was examined by UV spectrophotometer, liquid chromatography (LC) with diode array and fluorescence detection, H-1 and C-13-NMR spectrometry, LC-mass spectrometry (LC/MS/MS), ion chromatography (IC), and differential scanning calorimetry (DSC). The diagrams obtained from DSC analysis and the UV spectrum showed no detectable impurities. Likewise, no impurities were observed by LC analysis with both diode array and fluorescence detection. IC determination revealed negligible contamination of ZON with chloride of 0.020 +/- 0.005 % and nitrate of 0.016 +/- 0.006%. Zearalanone (ZAN) was identified as one of 2 minor (0.2%) impurities by LC/MS/MS. The H-1-NMR measurements revealed an additional peak, which has not been previously reported in the literature. It could be identified as part of the ZON spectrum as the signal arising from the phenolic proton attached to C-4. The manufacturer states an additional contamination with 0.2% methylene chloride, which could be confirmed to an extent of 0.1 % by 1H-NMR. Minor impurities, whose structures remain unknown, were discovered at 3.5 and <1 ppm. Total percentage of impurities based on NMR measurement was estimated not to exceed 1 %. A purity of 99.5% with a tolerance of +/-0.5% was finally attributed to the ZON studied in this project


Mycotoxin Zearalenone; Estrogenic Mycotoxins; Physicochemical Data; Deoxynivalenol; Toxins

Published in

2003, volume: 86, number: 4, pages: 722-728

Authors' information

Pettersson, Hans
Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management
Berner, D
Charlton, A
Hametner, C
Zollner, P
Welzig, E
Brereton, P
Krska, R
Josephs, R D
MacDonald, S
Kandler, W

UKÄ Subject classification

Veterinary Science
Food Science
Animal and Dairy Science

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