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Abstract

The World Organization for Animal Health (Office International des Epizooties, OIE) includes the diseases caused by foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV), as "Diseases Notifiable to the OIE". Foot-and-mouth disease (FMD) outbreaks have severe economical as well as social effects and cannot be differentiated from the diseases caused by the other two viruses on the basis of clinical symptoms. Efficient laboratory techniques are therefore required for detection and identification of the viruses causing similar vesicular symptoms in swine. A rapid method is described using padlock probes and microarrays to detect simultaneously and differentiate the three viruses in a single reaction, as well as providing serotype information in cases of VSV infection. The padlock probe/microarray assay detected successfully and identified 39 cDNA samples of different origin representing the three viruses. The results were in complete agreement with identities and serotypes determined previously. This novel virus detection method is discussed in terms of usefulness and further development. (C) 2007 Elsevier B.V. All rights reserved.

Keywords

foot-and mouth disease; swine vesicular disease; vesicular stomatitis; virus; padlock probes; microarray; rapid detection

Published in

Journal of Virological Methods
2007, volume: 143, number: 2, pages: 200-206
Publisher: ELSEVIER SCIENCE BV

SLU Authors

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science

Publication identifier

  • DOI: https://doi.org/10.1016/j.jviromet.2007.03.004

Permanent link to this page (URI)

https://res.slu.se/id/publ/15105