Decreased susceptibility to doxycycline associated with a 16S r-PINA gene mutation in Brachyspira hyodysenteriae

Abstract

The objective of this study was to assess whether nucleotide substitutions in the 16S rDNA sequence of selected Brachyspira hyodysenteriae isolates could explain differences in doxycycline minimal inhibitory concentrations (MICs). The main part of the 16S rRNA gene was sequenced and compared for 19 isolates with different doxycycline MICs. A mutation in the 16S rRNA gene at the position corresponding to 1058 in Escherichia coli has been shown to cause tetracycline resistance in other bacteria. In the B. hyodysenteriae sequences a G1058C mutation was found for all isolates with increased doxycycline MICs whereas all susceptible isolates had the wild type sequence. (C) 2007 Elsevier B.V. All fights reserved.

Keywords

antibiotic resistance; brachyspira hyodysenteriae; tetracycline; 16S rRNA; swine dysentery

Published in

Veterinary Microbiology
2007, volume: 123, number: 45660, pages: 245-248
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Pringle, Märit

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Fellström, Claes

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Johansson, Karl-Erik

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science

Publication identifier

• DOI: https://doi.org/10.1016/j.vetmic.2007.02.019

Permanent link to this page (URI)

https://res.slu.se/id/publ/15748