Abstract

The complete genome for a barley isolate of Wheat dwarf virus (WDV) from Tekirdaag, Turkey, WDV-Bar[TR], was isolated and sequenced. The genome was found to be 2739 nucleotides long, which is shorter than wheat-infecting WDV isolates, and with a genome organization typical for mastreviruses. The complete genome of WDV-Bar[TR] showed 83-84% nucleotide identity to wheat isolates of WDV, with the non-coding regions SIR and LIR least conserved (72-74% identity). The deduced amino acid sequences for Rep and RepA were most conserved (92-93%), while CP and MP were less conserved (87% and 79-80%, respectively). The identity to other mastrevirus species was significantly lower. In phylogenetic analyses, the WDV isolates formed a distinct clade, well separated from the other mastreviruses with the wheat isolates grouping closely together. Phylogenetic analyses of WDV-Bar[TR], the partial sequence for another Turkish barley isolate (WDV-Bar[TR2]) and published WDV sequences further supported the division of WDV into two distinct strains. The barley strain could also be divided into three subtypes based on relationships and geographic origin. This study shows the first complete published sequence for a barley isolate of WDV

Published in

Virus Genes
2007, Volume: 34, number: 3, pages: 359-366
Publisher: SPRINGER

SLU Authors

• Ramsell, Jon

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Kvarnheden, Anders

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science


Publication identifier

DOI: https://doi.org/10.1007/s11262-006-0029-0

Permanent link to this page (URI)

https://res.slu.se/id/publ/15986