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Research article - Peer-reviewed, 2007

Regeneration and genetic transformation of Hagenia abyssinica (Bruce) JF Gmel. (Rosaceae) with rolB gene

Feyissa T, Zhu LH, Negash L, Welander M

Abstract

The effects of growth regulators, wounding and antibiotics on regeneration of Hagenia abyssinica were investigated and the rolB gene was introduced into this species by Agrobacterium-mediated transformation. Regeneration was affected by type of growth regulators, wounding and antibiotics. Up to 100% regeneration could be obtained. Three transformed clones (T1, T2.1, T2.2), confirmed by PCR and Southern blot, were obtained only by excluding kanamycin from the selection medium 6 weeks after culture, followed by selection during shoot multiplication. RT-PCR revealed strong expression of rolB gene in shoots and roots of all the transgenic clones, but from leaf samples, it was detected only in T1. Rooting frequency was 77% (T1), 50% (T2.1), 57% (T2.2) and 0% for control shoots on growth regulator-free rooting medium

Published in

Plant Cell, Tissue and Organ Culture
2007, Volume: 88, number: 3, pages: 277-288
Publisher: SPRINGER

      SLU Authors

    • UKÄ Subject classification

      Horticulture
      Forest Science

      Publication identifier

      DOI: https://doi.org/10.1007/s11240-006-9200-5

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/16246