Alm, Gunnar
- Department of Molecular Biosciences, Swedish University of Agricultural Sciences
Abstract
It has been previously shown that antibodies contained in human plasma directed towards the Coxsackievirus B4 (CVB4)E2 capsid protein VP4 can enhance the CVBE2-induced production of IFN-alpha by peripheral blood mononuclear cells (PBMC). The aim of this study was to produce a VP4 fusion protein to investigate the role of the internal capsid protein VP4 and anti-VP4 antibodies in the plasma-dependent enhancement of CVB4E2 infection of PBMC. A fusion protein MBPVP4 containing the VP4 insert of CVB4E2 and a control fusion protein MBP-beta-gal-alpha, were produced in Escherichia coli K12 TB1. The CVB4E2 infection of PBMC was quantified by using a real time PCR method amplifying CVB4E2-negative strand RNA. IFN-alpha concentrations in culture supernatants were assayed by DELFIA. MBPVP4 but not MBP-beta-gal-alpha, preincubated with plasma inhibited the plasma-dependent enhancement of CVB4E2-induced production of IFN-alpha by PBMC. Human plasma samples, antibodies contained in plasma eluted from MBPVP4-coated plates, but not from MBP-beta-gal-alpha-coated plates, incubated with CVB4E2 enhanced the infection of PBMC and the production of IFN-alpha by infected cells. Together our results show that VP4 and anfi-VP4 antibodies play a role in the plasma-dependent enhancement of CVB4E2 infection of PBMC. (c) 2007 Elsevier B.V. All rights reserved.
Keywords
fusion protein; VP4; plasma; peripheral blood mononuclear cells; coxsackievirus B4
Published in
Virus Research
2007, volume: 125, number: 2, pages: 183-190
Publisher: ELSEVIER SCIENCE BV
SLU Authors
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1016/j.virusres.2007.01.001
Permanent link to this page (URI)
https://res.slu.se/id/publ/16360