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Research article - Peer-reviewed, 2007

Efficient mapping of mendelian traits in dogs through genome-wide association

Karlsson EK, Baranowska I, Wade CM, Salmon Hillbertz NHC, Zody MC, Anderson N, Biagi TM, Patterson N, Pielberg GR, Kulbokas EJ, Comstock KE, Keller ET, Mesirov JP, von Euler H, Kampe O, Hedhammar A, Lander ES, Andersson R, Andersson L, Lindblad-Toh K

Abstract

With several hundred genetic diseases and an advantageous genome structure, dogs are ideal for mapping genes that cause disease. Here we report the development of a genotyping array with similar to 27,000 SNPs and show that genome-wide association mapping of mendelian traits in dog breeds can be achieved with only similar to 20 dogs. Specifically, we map two traits with mendelian inheritance: the major white spotting ( S) locus and the hair ridge in Rhodesian ridgebacks. For both traits, we map the loci to discrete regions of <1 Mb. Fine-mapping of the S locus in two breeds refines the localization to a region of similar to 100 kb contained within the pigmentation-related gene MITF. Complete sequencing of the white and solid haplotypes identifies candidate regulatory mutations in the melanocyte-specific promoter of MITF. Our results show that genome-wide association mapping within dog breeds, followed by fine-mapping across multiple breeds, will be highly efficient and generally applicable to trait mapping, providing insights into canine and human health

Published in

Nature Genetics
2007, Volume: 39, number: 11, pages: 1321-1328
Publisher: NATURE PUBLISHING GROUP