Dida, Mulatu Geleta
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Abstract
Amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) were employed to examine the gene tic relationship between Guizotia taxa, to suggest the taxonomic status of some of these taxa, and to identify their diagnostic markers. Results from AFLPs and RAPDs share some features in common, both revealing G. scabra ssp. Schimperi as the most closely related taxon to G. abyssinica, and indicating that G. arborescens and G. zavattarii are the most divergent taxa. Most of the diagnostic markers revealed in this study were specific to G. arborescens and G. zavattarii. Our analysis suggests that G. scabra ssp. seabra, G. scabra ssp. schimperi, Chelelu and Ketcha are separate species. In this study, AFLP was found to be superior to RAPD in detecting genetic variation, in internal consistency of the data and in the fitness of its clusters to genetic similarity data. AFLPs revealed genetic relationship between Guizotia taxa that is more inline with the cytogenetic and hybridization studies than that revealed by RAPDs.
Keywords
AFLP; diagnostic markers; genetic; relationship; guizotia cass; RAPD
Published in
Plant Systematics and Evolution
2007, volume: 265, number: 45720, pages: 221-239
Publisher: SPRINGER WIEN
SLU Authors
Bryngelsson, Tomas
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1007/s00606-007-0521-6
Permanent link to this page (URI)
https://res.slu.se/id/publ/17092