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Conference paper2007

Segmentation of Cytoplasms of Cultured Cells

Allalou, Amin; Wählby, Carolina; van, de Rijke Frans; Jahangir, Tafrechi Roos; Raap, Anton


Cell cultures as well as cells in tissue always display a certain degree of variability, and measurements based on cell averages will miss important information contained in a heterogeneous population. This paper presents automated methods for segmentation of cells and cytoplasms. The segmentation results are applied to image based measurements of mitochondiral DNA (mtDNA) mutations in individual cells. Three different methods for segmentation of cytoplasms are compared and it is shown that automated cytoplasmic delineation can be performed 30 times faster than manual delineation, with an accuracy as high as 87%, compared to an inter observer variability of 79% at manual delineation

Published in


Swedish symposioum in Image Analysis 2007

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