Research article - Peer-reviewed, 2008
Effect of acaricides on the activity of glutathione transferases from the parasitic mite Sarcoptes scabiel
Molin, E. U.; Mattsson, J. G.Abstract
Glutathione transferases (GSTs) are a family of multifunctional enzymes with fundamental roles in cellular detoxication. Here we report the molecular characterization of 3 recombinant GSTs belonging to the mu- and delta-class from the parasitic mite Sarcoptes scabiei. Kinetic constants were determined, and the effect of acaricides, including organothio-phosphates, pyrethroid esters, a formamidine, a macrocyclic lactone, an organochlorine as well as a bridged diphenyl acaricide, on the activity of the GSTs were tested using 1-chloro-2,4-dinitrobenzene (CDNB) as model substrate. Our results showed that enzymes from the same class and with high amino acid sequence identity have significantly different kinetic properties. For instance, one mu-class GST lost more than 50% of its activity in the presence of one of the organothiophosphates while the activity of the second mu-class GST was only slightly reduced under identical conditions. Tertiary structure modulations indicated that structural differences were the crucial factor for the different kinetic patterns observed. Genome analysis showed that the two mu-class GSTs are organized in tandem in the S. scabiei genome. Taken together these results show that GSTs might be involved in the metabolism of acaricides in S. scabiei.Keywords
Sarcoptes scabiei; glutathione transferase; enzyme activity; acaricide; kinetic analysis; genome organizationPublished in
Parasitology2008, volume: 135, number: 1, pages: 115-123
Authors' information
Molin, Eva U. (Molin, Eva)
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Molin, Eva U. (Molin, Eva)
National Veterinary Institute (SVA)
Mattsson, Jens
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Mattsson, Jens
National Veterinary Institute (SVA)
UKÄ Subject classification
Animal and Dairy Science
Pathobiology
Publication Identifiers
DOI: https://doi.org/10.1017/S0031182007003472
URI (permanent link to this page)
https://res.slu.se/id/publ/17436