MZF6D, a novel KRAB zinc-finger gene expressed exclusively in meiotic male germ cells

Abstract

Spermatogenesis takes place in the seminiferous tubule in the testes and culminates in the production of spermatozoa (male gametes). Here we report the identification of a novel mouse zinc-finger gene, MZF6D, which is selectively expressed in meiotic spermatocytes. The MZF6D protein contains an N-terminally located repressor domain, a KRAB domain, followed by at least seven successive Kruppel zinc-finger motifs. The KRAB domain of MZF6D, which consists of a KRAB A box and the newly identified KRAB C box, has previously been shown to interact with TIF1beta, which is the common corepressor of all KRAB zinc-finger proteins. Northern blot analysis shows that the expression of MZF6D is restricted to testes. This was confirmed by RT-PCR analysis of a panel of mouse tissues. In situ hybridization of sections from adult mouse testes localizes the expression to meiotic spermatocytes, suggesting a specific role for MZF6D in the regulation of spermatogenesis

Published in

DNA and Cell Biology
2003, volume: 22, number: 8, pages: 489-496
Publisher: MARY ANN LIEBERT INC PUBL

SLU Authors

• Åbrink, Magnus

  • Department of Molecular Biosciences, Swedish University of Agricultural Sciences
    

Publication identifier

• DOI: https://doi.org/10.1089/10445490360708892

Permanent link to this page (URI)

https://res.slu.se/id/publ/1819