Abstract

Mating type ratios and SSR marker analysis were used to study the genetic structure of Phaeosphaeria nodorum, the causal agent of glume blotch in wheat. The study was based on leaf collections in five fields located in different regions in Sweden. In total 302 isolates of P. nodorum were obtained from 203 sampling sites (including eight ascospore isolates). Three strong indications of sexual recombination were found: (i) the two mating types were present at a 1:1 ratio; (ii) the genetic structure was diverse, with many unique genotypes, and 69 of the 93 genotypes were only found once; and (iii) random association of alleles indicated that genetic recombination was frequent. However, asexual reproduction could not be excluded since identical genotypes were found within the fields. The fungal population had experienced a demographic bottleneck, as indicated by a low ratio of number of alleles to microsatellite size range (M-value) of 0.5.

Keywords

demographic bottleneck; genetic diversity; mating type; sexual recombination; Stagonospora nodorum; wheat glume blotch

Published in

Plant Pathology
2008, Volume: 57, number: 4, pages: 634-641

SLU Authors

• Edin, Eva

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Olson, Åke

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Högberg, Nils

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Djurle, Annika

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Yuen, Jonathan

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science
Food Science


Publication identifier

DOI: https://doi.org/10.1111/j.1365-3059.2008.01826.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/18900