Abstract

Arbuscular mycorrhizal (AM) fungi and their bacterial associates are essential living components of the soil microbiota. From a total of 385 bacteria previously isolated from spores of AM fungi (AMB), 10 were selected based on ability to inhibit growth of plant pathogens. Effects of these isolates on AM fungal colonization, plant growth in potato (Solanum ruberosum L.) and inhibition of pathogens was investigated. AM fungal root colonization of potato was 7-fold higher in the presence of the Pseudomonas FWC70 isolate in a greenhouse and was 6-9-fold higher in the presence of the three isolates Pseudomonas FWC70, Stenotrophomonas FWC94 and Arthrobacter FWC110 in an outdoor pot experiment. Several growth traits of potato were stimulated by the Pseudomonas isolates FWC16, FWC30 and FWC70 and by the Stenotrophomonas isolate FWC14. All three Pseudomonas isolates showed inhibition against Erwinia carotovora, Phytophthora infestans and Verticillium dahliae but Stenotrophomonas isolates were variable. Protease(s), siderophores and indole acetic acid were produced by all isolates. Chitinase(s) were produced by all Stenotrophomonas and phosphate-solubilizing activity by all Pseudomonas isolates, the Stenotrophomonas FWC14 isolate and the Arthrobacter FWC110 isolate. We conclude that some AMB are multifunctional and production of extracellular enzymes and bioactive compounds are likely mechanisms for their multifunctional activities. Our results show that some AMB are likely to contribute to the often described ability of AM fungi to inhibit pathogens, acquire mineral nutrients and modify plant root growth. (C) 2008 Elsevier Ltd. All rights reserved.

Keywords

Arbuscular mycorrhizal fungi; Glomus; Plant pathogen; Potato; Root colonization; Spore-associated bacteria

Published in

Soil Biology and Biochemistry
2008, Volume: 40, number: 10, pages: 2494-2501
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

• Bharadwaj, Dharam

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Lundquist, Per-Olof

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Alström, Sadhna

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Horticulture
Agricultural Science
Environmental Sciences related to Agriculture and Land-use


Publication identifier

DOI: https://doi.org/10.1016/j.soilbio.2008.06.012

Permanent link to this page (URI)

https://res.slu.se/id/publ/19341