Influence of seminal plasma on the kinematics of boar spermatozoa during freezing

Abstract

Sperm motolity is, for its relation to cell viability and fertility, a central component of the spermiogram, where consideration of motion patterns allows discrimination of sub-populations among boar spermatozoa. Extension and cryo-preservation imposes changes in these patterns in connection to handling, additives, temperature changes and the removal of boar seminal plasma (BSP) which apparently makes spermatozao susceptible to xodative stress, thus affecting survival and motility post-thaw. Detailed kinematic analyses during sperm cooling are sparse, particularly when considering the instrumentation and settings used for analyses, the effect of extenders, and of the BSP the processed spermatozoa are exposed to. Spermatozoa present in the first collectable 10 mL of the sperm-rich fraction of the ejaculate (portion 1. P1-BSP), have shown an increased ability to sustain motility during and after cryo-preservation than spermatozoa immersed in the rest of the ejaculate (portion 2, P2). When P2-spermatozoa were cleansed from their BSP and exposed for 60 min to pooled P1-BSP, their motility post-thaw increased to similar levels as P1-spermatozoa. This BSP-influence is sire-dependent, presumably related to the protein concentration in the different ejaculate poriton,s andapparently unrealted to changes in membrane integrity or membrane stability through conventiona, controlled cooling. (C) 2008 Elsevier Inc. All rights reserved.

Keywords

Sperm kinematics; CASA; Sperm populations; Seminal plasma; Boar

Published in

Theriogenology
2008, volume: 70, number: 8, pages: 1242-1250

SLU Authors

• Rodriguez, Heriberto

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Saravia-Ramos, Fernando

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Wallgren, Margareta

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Quality Genetics

UKÄ Subject classification

Food Science
Animal and Dairy Science
Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1016/j.theriogenology.2008.06.007

Permanent link to this page (URI)

https://res.slu.se/id/publ/19954