Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen

Abstract

The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E (TM)). Sperm motility, sperm chromatin structure, membrane integrity and mitorchondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E (TM) significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI. (c) 2008 Elsevier B.V. All rights reserved.

Keywords

Frozen-thawed stallion semen; Colloid separation; CASA; Kinematics; Sperm morphology (ASMA); Membrane integrity; Mitochondrial function; SCSA; Sperm subpopulations

Published in

Animal Reproduction Science
2009, Volume: 114, number: 1-3, pages: 193-202

SLU Authors

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Rodriguez, Heriberto

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science


Publication identifier

DOI: https://doi.org/10.1016/j.anireprosci.2008.08.025

Permanent link to this page (URI)

https://res.slu.se/id/publ/19975