Abstract

Flowering plants were regenerated from in vitro grown leaves of miniature rose (Rosa hybrida L. 'Linda') via somatic embryogenesis. Sufficient amounts of callus were produced on in vitro leaves of 8 cultivars ('Leonie', 'Linda', 'Sonja', 'Toledo', 'Tiffany', 'Mette', 'Etna' and 'Andromeda') incubated on MS media fortified with either 45.2 or 90.5 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) for 6 weeks. Except for 'Tiffany' and 'Andromeda', embryogenic callus was induced on the callus of all cultivars incubated on MS media supplemented with different concentrations of zeatin or thidiazuron (TDZ). The highest frequency of primary somatic embryogenesis (30 %) was achieved with 'Sonja' on medium containing 22.7 mu M TDZ, but the embryos from 'Sonja' failed to develop into plants. Only the somatic embryos of 'Linda' developed into flowering plants thus further work was carried out with this cultivar. The frequency of primary somatic embryogenesis in 'Linda' was 3.3 % on medium containing either 45.6 mu M zeatin or 45.4 mu M TDZ. The highest proliferation rate (3.8-fold) and the highest frequency of embryo maturation (76.6 %) were obtained on 1/2 MS medium fortified with 7.57 mu M abscisic acid. Germination and subsequent shoot development on mature somatic embryos occurred in a medium containing 1.42 mu M 1-naphthaleneacetic acid (NAA), 17.76 mu M 6-benzylaminopurine (BAP) and 1.44 mu M gibberellic acid (GA(3)). Shoots were rooted on 1/2 MS medium supplemented with 5.7 mu M 3-indole acetic acid (IAA) and 9.84 mu M indole-3-butyric acid (IBA) and transferred to the greenhouse. The plants flowered after about 10 weeks and were morphologically indistinguishable from the original plant.

Keywords

callus; plant growth regulator; plant regeneration; rose; somatic embryos

Published in

European Journal of Horticultural Science
2008, volume: 73, number: 3, pages: 111-117
Publisher: EUGEN ULMER GMBH CO

Authors' information