Gyarmati, Peter
- National Veterinary Institute (SVA)
Abstract
A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods
Published in
Journal of Clinical Microbiology
2008, volume: 46, number: 5, pages: 1747-1751
Publisher: AMER SOC MICROBIOLOGY
SLU Authors
Zohari, Siamak
- National Veterinary Institute (SVA)
Belak, Sandor
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Animal and Dairy Science
Veterinary Science
Publication identifier
- DOI: https://doi.org/10.1128/JCM.02292-07
Permanent link to this page (URI)
https://res.slu.se/id/publ/20425