Abstract

The porcine circovirus type 2 (PCV2) genome encodes three major open reading frames (ORFs) encoding the replicase proteins (ORF1), the viral capsid protein (ORF2), and a protein with suggested apoptotic activity (ORF3). Previous phylogenetic analyses of complete genome sequences of PCV2 from GenBank have demonstrated 95-100% intra-group nucleotide sequence identity. However, although these isolates were readily grouped into clusters and clades, there was no correlation between the occurrence of specific PCV2 genotypes and the geographic origin or health status of the pig. In the present study, a unique dataset from a field study spanning the years pre and post the recognition of postweaning multisystemic wasting syndrome (PMWS) in Sweden was utilized. Using this dataset it was possible to discriminate three Swedish genogroups (SG1-3) of PCV2, of which SG1 was recovered from a pig on a healthy farm ten years before the first diagnosis of PMWS in Sweden. The SG1 PCV2/ORF2 gene sequence has been demonstrated to exhibit a high genetic stability over time and has subsequently only been demonstrated in samples from pigs on nondiseased farms. In contrast, SG2 was almost exclusively found on farms that had only recently broken down with PMWS whereas the SG3 genogroup predominated in pigs from PMWS-affected farms. These results further support the results obtained from earlier in vitro and in vivo experimental models and suggest the association of specific PCV2 genogroups with diseased and nondiseased pigs in the field.

Keywords

PMWS; PCV2; circovirus genogroups; capsid protein; apoptin

Published in

Virus Genes
2008, Volume: 36, number: 3, pages: 509-520
Publisher: SPRINGER

SLU Authors

• Timmusk, Sirje

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Wallgren, Per

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • National Veterinary Institute (SVA)

• Hasslung Wikström, Frida

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Fuxler, Lisbeth

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Berg, Mikael

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Fossum, Caroline

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science


Publication identifier

DOI: https://doi.org/10.1007/s11262-008-0217-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/20453