Research article - Peer-reviewed, 2009
Integrated Analysis of Transcript, Protein and Metabolite Data To Study Lignin Biosynthesis in Hybrid Aspen
Bylesjoe, Max; Nilsson, Robert; Srivastava, Vaibhav; Gronlund, Andreas; Johansson, Annika I.; Jansson, Stefan; Karlsson, Jan; Moritz, Thomas; Wingsle, Gunnar; Trygg, JohanAbstract
Tree biotechnology will soon reach a mature state where it will influence the overall supply of fiber, energy and wood products. We are now ready to make the transition from identifying candidate genes, controlling important biological processes, to discovering the detailed molecular function of these genes on a broader, more holistic, systems biology level. In this paper, a strategy is outlined for informative data generation and integrated modeling of systematic changes in transcript, protein and metabolite profiles measured from hybrid aspen samples. The aim is to study characteristics of common changes in relation to genotype-specific perturbations affecting the lignin biosynthesis and growth. We show that a considerable part of the systematic effects in the system can be tracked across all platforms and that the approach has a high potential value in functional characterization of candidate genes.Keywords
Combined profiling; O2PLS; Chemometrics; Populus; Lignin biosynthesisPublished in
Journal of Proteome Research2009, volume: 8, number: 1, pages: 199-210
Authors' information
Bylesjö, Max
Umeå University
Nilsson, Robert
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Srivastava, Vaibhav
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Grönlund, Andreas
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Jansson, Stefan
Karlsson, Jan
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Trygg, Johan
UKÄ Subject classification
Forest Science
Publication Identifiers
DOI: https://doi.org/10.1021/pr800298s
URI (permanent link to this page)
https://res.slu.se/id/publ/20805