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Research article2007Peer reviewed

Determination of molar absorptivity coefficients for major type-B trichothecenes and certification of calibrators for deoxynivalenol and nivalenol

Krska R, Schubert-Ullrich P, Josephs RD, Emteborg H, Buttinger G, Pettersson H, van Egmond HP, Schothorst RC, MacDonald S, Chan D


This paper presents results from the European Commission-funded project Doncalibrant, the objective of which was to produce calibrators with certified mass fractions of the Fusarium toxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-Ac-DON), 15-acetyldeoxynivalenol (15-Ac-DON), and nivalenol (NIV), in acetonitrile. The calibrators, available in ampoules, were sufficiently homogeneous, with between-bottle variations (s(bb)) of less than 2%. Long-term stability studies performed at four different temperatures between -18 and 40 degrees C revealed no significant negative trends (at a confidence level of 95%). Molar absorptivity coefficients (in L mol(-1) cm(-1)) were determined for all four toxins (DON: 6805 +/- 126, NIV: 6955 +/- 205, 3-Ac-DON: 6983 +/- 141, 15-Ac-DON: 6935 +/- 142) on the basis of a mini-interlaboratory exercise. The overall uncertainty of the calibrators' target values for DON and NIV were evaluated on the basis of gravimetric preparation data and include uncertainty contributions from possible heterogeneity, storage, and transport. The Doncalibrant project resulted in the production of calibrators for DON (IRMM-315) and NIV (IRMM-316) in acetonitrile with certified mass fractions of 25.1 +/- 1.2 mu g g(-1) and 24.0 +/- 1.1 mu g g(-1), respectively. Both CRMs became commercially available from the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) at the beginning of 2007


deoxynivalenol; fungal toxins; Hyphomycetes; calibration; determination; instruments; mycotoxins; nivalenol; quantitative analysis; trichothecenes; vomitoxin

Published in

Analytical and Bioanalytical Chemistry
2007, Volume: 388, number: 5-6, pages: 1215-1226

    UKÄ Subject classification

    Veterinary Science
    Animal and Dairy Science
    Food Science

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