Pettersson, Hans
- Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
Abstract
This paper presents results from the European Commission-funded project Doncalibrant, the objective of which was to produce calibrators with certified mass fractions of the Fusarium toxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-Ac-DON), 15-acetyldeoxynivalenol (15-Ac-DON), and nivalenol (NIV), in acetonitrile. The calibrators, available in ampoules, were sufficiently homogeneous, with between-bottle variations (s(bb)) of less than 2%. Long-term stability studies performed at four different temperatures between -18 and 40 degrees C revealed no significant negative trends (at a confidence level of 95%). Molar absorptivity coefficients (in L mol(-1) cm(-1)) were determined for all four toxins (DON: 6805 +/- 126, NIV: 6955 +/- 205, 3-Ac-DON: 6983 +/- 141, 15-Ac-DON: 6935 +/- 142) on the basis of a mini-interlaboratory exercise. The overall uncertainty of the calibrators' target values for DON and NIV were evaluated on the basis of gravimetric preparation data and include uncertainty contributions from possible heterogeneity, storage, and transport. The Doncalibrant project resulted in the production of calibrators for DON (IRMM-315) and NIV (IRMM-316) in acetonitrile with certified mass fractions of 25.1 +/- 1.2 mu g g(-1) and 24.0 +/- 1.1 mu g g(-1), respectively. Both CRMs became commercially available from the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) at the beginning of 2007.
Keywords
mycotoxin; certified reference material; calibrator
Published in
Analytical and Bioanalytical Chemistry
2007, volume: 388, number: 45783, pages: 1215-1226
Publisher: SPRINGER HEIDELBERG
SLU Authors
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Food Science
Publication identifier
- DOI: https://doi.org/10.1007/s00216-007-1369-x
Permanent link to this page (URI)
https://res.slu.se/id/publ/22243