Abstract

The yeast Saccharomyces cerevisiae has two closely related plasma membrane syntaxins, Sso1p and Sso2p, which together provide an essential function in vegetative cells. However, Sso1p is also specifically needed during sporulation, and this function cannot be provided by Sso2p. We have used fusions between SSO1 and SSO2 to map the sporulation-specific function of SSO1. We found that the two N-terminal a-helices Ha and Hb of Sso1p are important for sporulation, since it is reduced 8-fold for fusions where Ha and Hb are derived from Sso2p. In contrast, the C-terminal half of Sso1p does not seem to be specifically required for sporulation. Surprisingly, we further found that the 3'-untranslated region of SSO1 is essential for sporulation. Western blots failed to reveal a preferential expression of Sso1p in sporulating cells, indicating that effects on gene expression are unlikely to explain why the SSO1 3'-untranslated region is needed for sporulation

Keywords

Sso1p; Sso2p; Sporulation; Syntaxin; 3'-Untranslated Region; Yeast

Published in

Current Genetics
2004, volume: 45, pages: 76-82

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