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Research article - Peer-reviewed, 2007

Evaluation of methods for single hair DNA amplification

Bjornerfeldt S, Vila C


Because of the low amount of DNA in single hairs, it may be difficult to obtain reliable genotypes for forensic and conservation genetics studies. We therefore compared different methods for reliably genotyping single hair samples. Our results indicate that preliminary whole genome amplification can increase the likelihood of successfully genotyping a single hair compared to other commonly used protocols. The difference between the methods is small for single locus comparisons, but it becomes more important in multi-locus comparisons. The economic and time costs of the whole genome amplification may prevent its large-scale use in non-invasive monitoring programs. Nevertheless, it may be a very useful approach for the analysis of especially valuable samples

Published in

Conservation Genetics
2007, volume: 8, number: 4, pages: 977-981
Publisher: SPRINGER

Authors' information

Björnerfeldt, Susanne
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Vila`, Carles

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