Abstract

This research aimed to describe the genetic and phenotypic diversity of 74 Spanish Brachyspira hyodysenteriae field isolates, to establish epidemiological relationships between the isolates and to confirm the presence of tiamulin-resistant isolates in Spain. For these purposes, we performed biochemical tests in combination with diagnostic PCR analysis for the identification of Brachyspira spp. and for detection of the smpA/smpB gene. We also used antimicrobial susceptibility tests, random amplified polymorphic DNA (RAPD) and a new pulsed-field gel electrophoresis (PFGE) protocol. The combination of RAPD and PFGE allowed the study of epidemiological relationships. Both indole-negative and tiamulin-resistant isolates of B. hyodysenteriae are reported in Spain for the first time. The genetic analyses indicated a relationship between these Spanish isolates and indole-negative isolates previously obtained from Germany and Belgium.

Keywords

Brachyspira hyodysenteriae; characterization; indole negative; PFGE; RAPD

Published in

Epidemiology and Infection
2010, Volume: 138, number: 1, pages: 76-85

SLU Authors

• Pringle, Märit

  • Department of Biomedical Science and Veterinary Public Health, Swedish University of Agricultural Sciences
    

• Fellström, Claes

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science


Publication Identifiers

DOI: https://doi.org/10.1017/S0950268809002817

Permanent link to this page (URI)

https://res.slu.se/id/publ/25810