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Conference abstract - Peer-reviewed, 2009

AUTOMATIC DIGITAL IMAGE ANALYSIS SYSTEM (CELLAVISION DM96) AS A TOOL FOR CANINE LEUKOCYTE DIFFERENTIAL COUNTS

Lilliehöök, Inger; Tvedten, Harold

Abstract

CellaVision DM96 (CellaVision AB, Lund, Sweden) is a computerized microscope that automatically make digital photos of cell and structures in stained blood smears and pre-classified them in 16 groups. The aim was to evaluate if CellaVision DM96 could be a useful tool for leukocyte differential counts in samples from dogs. Blood smears from107 fresh samples from canine patients were stained with May Grunewald Giemsa stain and analysed with DM96. The DM96 was set to count 125 leukocytes. After analysis the pre-classified cells were nicely displayed with photographs of very good quality. It was possible to magnify the pictures, compare different cell groups and easy to change cell classification. In average 174 cells/structures were detected on the smears. The over all agreement of DM96 pre-classification and after reclassification was 78%. In average 38 cells/structures per smear was to be reclassified. Pre-classification was in agreement with final results for 91% of the lymphocytes, 88% of the neutrophils, 69% of the monocytes and 97% of the NRBCs. DM96 did, however, only identify 37% of the eosinophils and 2% of the basophils. CellaVision DM96 has very high demands on staining quality and size/form of the blood smear. In some samples with weak staining many neutrophils were erroneously classified as artefacts. DM96 was easy to work with and showed excellent pictures of all cells in spite of the fact that the instrument only has human software The pre-classification facilitated the differential count considerably especially in samples with abnormal cells or leukopenia

Published in

Veterinary Clinical Pathology
2009, Volume: 38, number: S1

Conference

Annual Meeting of ESVCP/ECVCP