Lipid peroxidation, assessed with BODIPY-C-11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like changes

Ortega, Ferrusola Cristina; Gonzalez, Fernandez L; Morrell, Jane; Salazar, Sandoval C; Macias, Garcia B; Rodriguez, Heriberto; Tapia, JA; Pena, FJ

doi:10.1530/REP-08-0484

Research article2009Peer reviewedOpen access

Lipid peroxidation, assessed with BODIPY-C-11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like changes

Ortega, Ferrusola Cristina; Gonzalez, Fernandez L; Morrell, Jane; Salazar, Sandoval C; Macias, Garcia B; Rodriguez, Heriberto; Tapia, JA; Pena, FJ

Abstract

Lipid peroxidation (LPO) of stallion spermatozoa was assessed in fresh semen and in samples of the same ejaculates after freezing and thawing. Particular attention was paid to individual differences in the susceptibility to LPO and its possible relationship with freezability. Innate levels of LPO were very low in fresh spermatozoa but increased after thawing, a change that was largely stallion-dependent. The level of LPO in fresh spermatozoa was not correlated with that of the thawed spermatozoa. Negative correlations existed between LPO and intact membranes post-thaw (r= -0.789, P

Published in

Reproduction
2009, volume: 138, number: 1, pages: 55-63

SLU Authors

Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Rodriguez, Heriberto
- Department of Clinical Sciences, Swedish University of Agricultural Sciences

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science

Publication identifier

DOI: https://doi.org/10.1530/REP-08-0484

Permanent link to this page (URI)

https://res.slu.se/id/publ/26732