Research article - Peer-reviewed, 2009
Species-specific primers for predation studies of the pollen beetle, Meligethes aeneus (Coleoptera, Nitidulidae)
Cassel-Lundhagen, Anna; Oberg, Sandra; Hogfeldt, Carol; Ekbom, BarbaraAbstract
Species-specific primers were developed for the pollen beetle (a pest in oilseed rape) for studies of predation by natural insect predators. Two forward and three reverse primers were designed within the mitochondrial COI gene and used in combination to amplify fragments in the size range of 163-290 bp. Remains of pollen beetle DNA were consistently detected in Pardosa spiders up to 24 h after ingestion but dropped drastically at 48 h. These primers will facilitate studies on biological control of this oilseed rape pest. Detection time was not correlated with fragment length as might be expected as the DNA gradually degrades into progressively shorter fragments over time.Keywords
biological control; Meligethes neneus; oilseed rape; Pardosa; predation; species-specific primersPublished in
Molecular Ecology Resources2009, volume: 9, number: 4, pages: 1132-1134
Authors' information
Swedish University of Agricultural Sciences, Department of Ecology
Öberg, Sandra
Swedish University of Agricultural Sciences, Department of Ecology
Swedish University of Agricultural Sciences, Department of Ecology
Ekbom, Barbara
Swedish University of Agricultural Sciences, Department of Ecology
UKÄ Subject classification
Environmental Sciences related to Agriculture and Land-use
Agricultural Science
Publication Identifiers
DOI: https://doi.org/10.1111/j.1755-0998.2009.02540.x
URI (permanent link to this page)
https://res.slu.se/id/publ/26802