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Research article2009Peer reviewedOpen access

Species-specific primers for predation studies of the pollen beetle, Meligethes aeneus (Coleoptera, Nitidulidae)

Cassel-Lundhagen, Anna; Oberg, Sandra; Hogfeldt, Carol; Ekbom, Barbara


Species-specific primers were developed for the pollen beetle (a pest in oilseed rape) for studies of predation by natural insect predators. Two forward and three reverse primers were designed within the mitochondrial COI gene and used in combination to amplify fragments in the size range of 163-290 bp. Remains of pollen beetle DNA were consistently detected in Pardosa spiders up to 24 h after ingestion but dropped drastically at 48 h. These primers will facilitate studies on biological control of this oilseed rape pest. Detection time was not correlated with fragment length as might be expected as the DNA gradually degrades into progressively shorter fragments over time.


biological control; Meligethes neneus; oilseed rape; Pardosa; predation; species-specific primers

Published in

Molecular Ecology Resources
2009, Volume: 9, number: 4, pages: 1132-1134

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        • UKÄ Subject classification

          Environmental Sciences related to Agriculture and Land-use
          Agricultural Science

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