Abstract

Rhamnogalacturonan II (RG-II) is a structurally complex cell wall pectic polysaccharide. Despite its complexity, both the structure of RG-II and its ability to dimerise via a borate diester are conserved in vascular plants suggesting that RG-II has a fundamental role in primary cell wall organisation and function. The selection and analysis of new mutants affected in RG-II formation represents a promising strategy to unravel these functions and to identify genes encoding enzymes involved in RG-II biosynthesis. In this paper, a novel fingerprinting strategy is described for the screening of RG-II mutants based on the mild acid hydrolysis of RG-II coupled to the analysis of the resulting fragments by mass spectrometry. This methodology was developed using RG-II fractions isolated from citrus pectins and then validated for RG-II isolated from the Arabidopsis mur1 mutant and irx10 irx10-like double mutant.

Keywords

Cell wall mutants; Fingerprinting; Mass spectrometry; Rhamnogalacturonan II

Published in

Planta
2009, Volume: 230, number: 5, pages: 947-957

SLU Authors

• Marchant, Alan

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • University of Southampton

UKÄ Subject classification

Forest Science


Publication identifier

DOI: https://doi.org/10.1007/s00425-009-0996-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/26845