Research article - Peer-reviewed, 2009
An Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and Synthesis
Petersson, Sara V.; Johansson, Annika I.; Kowalczyk, Mariusz; Makoveychuk, Alexander; Wang, Jean Y.; Moritz, Thomas; Grebe, Markus; Benfey, Philip N.; Sandberg, Goran; Ljung, KarinAbstract
Local concentration gradients of the plant growth regulator auxin (indole-3-acetic acid [IAA]) are thought to instruct the positioning of organ primordia and stem cell niches and to direct cell division, expansion, and differentiation. High-resolution measurements of endogenous IAA concentrations in support of the gradient hypothesis are required to substantiate this hypothesis. Here, we introduce fluorescence-activated cell sorting of green fluorescent protein-marked cell types combined with highly sensitive mass spectrometry methods as a novel means for analyses of IAA distribution and metabolism at cellular resolution. Our results reveal the presence of IAA concentration gradients within the Arabidopsis thaliana root tip with a distinct maximum in the organizing quiescent center of the root apex. We also demonstrate that the root apex provides an important source of IAA and that cells of all types display a high synthesis capacity, suggesting a substantial contribution of local biosynthesis to auxin homeostasis in the root tip. Our results indicate that local biosynthesis and polar transport combine to produce auxin gradients and maxima in the root tip.Published in
Plant Cell2009, volume: 21, number: 6, pages: 1659-1668
Authors' information
Petersson, Sara
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Kowalczyk, Mariusz
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Makoveychuk, Alexander
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Wang, Jean Y.
Duke University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Grebe, Markus
Umeå University
Benfey, Philip
Sandberg, Göran
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
UKÄ Subject classification
Forest Science
Publication Identifiers
DOI: https://doi.org/10.1105/tpc.109.066480
URI (permanent link to this page)
https://res.slu.se/id/publ/26859