Review article - Peer-reviewed, 2009
New developments in the diagnosis of avian influenzaBelak, S.; Kiss, I.; Viljoen, G. J.
AbstractAvian influenza has become a serious concern from both veterinary and public health points of view. National and international organisations, veterinary health authorities, research institutions, diagnostic laboratories and field services make enormous efforts worldwide to detect, combat and prevent this important disease. Accordingly, the standard diagnostic protocols are being supported by a wide variety of molecular detection techniques, including improved polymerase chain reaction assays, microarray-based detection and characterisation methods, very rapid sequencing, simple pen-side tests and other on-site approaches. These recently developed 'closer to the field' methods allow rapid detection of influenza viruses and the identification of pathogenicity variants. However, in order to harmonise the diagnosis worldwide, attention has to be paid to the validation and standardisation of these technologies, to avoid erroneous interpretation of assay results, and, consequently, inappropriate epidemiological measures.This review gives an overview of the current and potential future developments related to avian influenza diagnostics.
KeywordsAvian influenza; Isothermal amplification; Microarray; Molecular diagnosis; Pathotyping; Pen-side test; Polymerase chain reaction; Proximity ligation; Rapid sequencing; Standardisation
Published inRevue Scientifique et Technique- Office International des Epizooties
2009, volume: 28, number: 1, pages: 233-243
Belák, Sándor (Belak, Sandor)
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
National Veterinary Institute (SVA)
Viljoen, Gerrit J.
UKÄ Subject classification
Animal and Dairy Science
URI (permanent link to this page)