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Research article - Peer-reviewed, 2009

Investigation of T-cell responses and viral mRNA persistence in lymph nodes of pigs infected with porcine rubulavirus

Cuevas, J. Sandra; Rodriguez-Ropon, A.; Kennedy, Seamus; Moreno-Lopez, Jorge; Berg, Mikael; Hernandez-Jauregui, Pablo

Abstract

Selected lymphocyte subpopulations were studied and the distribution of viral mRNA were investigated during acute and persistent porcine rubulavirus (PoRV-LPMV) infection in Vietnamese pot-bellied pigs. Six pigs infected with PoRV-LPMV at 17 days of age exhibited clinical signs 7-10 days post-inoculation (pi). One infected piglet died 11 days pi while the other five recovered around day 13 pi and survived until euthanasia on day 277 pi. Increased numbers of CD8+, CD4+ and CD2+ T cells were detected during the acute phase of infection while CD8+ cells were elevated throughout the infection, including during the persistent stage. Specific antibodies against the haemagglutinin-neuraminidase protein of PoRV-LPMV were detected during persistent infection. Although infectious virus could not be recovered from tissues from any of the infected pigs at necropsy 277 days pi, PoRV-LPMV mRNA was detected in lymph nodes, pancreas and central nervous system using a nested polymerase chain reaction technique. Continued lymphocyte interaction with viral RNA may be an important factor in promoting cellular and humoral responses during persistent PoRV-LPMV infection. (C) 2008 Elsevier B.V. All rights reserved.

Keywords

Porcine rubulavirus; Persistent infection; Viral mRNA; T-cells; Immune response; Pigs

Published in

Veterinary Immunology and Immunopathology
2009, Volume: 127, number: 1-2, pages: 148-152