Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing

Abstract

Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample. The fruit body is identified as Gloeophyllum sepiarium, the mycelium is still unidentified but has different T-RFLP pattern. It means that the fruit body may not always be responsible for all, or parts of the degradation of wood. The fungi samples from Estonian all showed to be Serpula lacrymans when comparing the T-RFLP patterns with sequences from NCBI (National Center for Biotechnology Information) gene bank. Before the analysis it was suggested that it could be either of the S. lacrymans or S. himantioides. The S. lacrymans from Sweden do not have the same T-RFLP pattern as the Estonian S. lacrymans. The Coniophora species cultivated in lab have been sequenced and have the same or a very close agreement in sequence. They all matched C. puteana when the sequences were compared to other sequences in the NCBI gene bank

Keywords

Above ground; Brown rot; Coniophora; Colonization; Fungi; PCR; Sequencing; Serpula; T-RFLP; Wood

Conference

IRG, International Research group on wood preservation

SLU Authors

• Råberg, Ulrika

  • Department of Forest Products, Swedish University of Agricultural Sciences
    

• Högberg, Nils

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Land, Carl Johan

  • Department of Forest Products, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Forest Science

Permanent link to this page (URI)

https://res.slu.se/id/publ/2854