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Research article - Peer-reviewed, 2004

Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation

Pena FJ, Johannisson A, Wallgren M, Rodriguez-Martinez H


We investigated the effect of supplementing extended boar semen with different amounts of hyaluronan (HA) prior to freezing on post-thaw sperm characteristics. Using a split sample design, the effect of HA at a final concentration of 500 or 1000 mug/ml semen on post-thaw motility parameters, and membrane lipid architecture status assessed by merocyanine-540/YOPRO-1 and flow cytometry were evaluated. HA-supplementation improved motility parameters (P < 0.05 to P < 0.001) and decreased the percentage of hyperactivated spermatozoa (P < 0.05). HA-supplemented samples had more spermatozoa showing high lipid membrane stability as assessed with merocyanine-540. In conclusion, HA appeared to preserve post-thaw spermatozoa viability in vitro and maintained membrane stability after cryopreservation. (C) 2003 Elsevier Inc. All rights reserved

Published in

2004, Volume: 61, number: 1, pages: 63-70