Abstract

We investigated the effect of supplementing extended boar semen with different amounts of hyaluronan (HA) prior to freezing on post-thaw sperm characteristics. Using a split sample design, the effect of HA at a final concentration of 500 or 1000 mug/ml semen on post-thaw motility parameters, and membrane lipid architecture status assessed by merocyanine-540/YOPRO-1 and flow cytometry were evaluated. HA-supplementation improved motility parameters (P < 0.05 to P < 0.001) and decreased the percentage of hyperactivated spermatozoa (P < 0.05). HA-supplemented samples had more spermatozoa showing high lipid membrane stability as assessed with merocyanine-540. In conclusion, HA appeared to preserve post-thaw spermatozoa viability in vitro and maintained membrane stability after cryopreservation. (C) 2003 Elsevier Inc. All rights reserved

Published in

Theriogenology
2004, Volume: 61, number: 1, pages: 63-70
Publisher: ELSEVIER SCIENCE INC

SLU Authors

• Johannisson, Anders

  • Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences
    

• Rodriguez, Heriberto

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

Publication Identifiers

DOI: https://doi.org/10.1016/S0093-691X(03)00181-X

Permanent link to this page (URI)

https://res.slu.se/id/publ/2958