Single Layer Centrifugation of Stallion Spermatozoa through Androcoll (TM)-E does not Adversely Affect their Capacitation-Like Status, as Measured by CTC Staining

Abstract

This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation-like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6 degrees C. Sperm capacitation-like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation-like status between colloid-selected and non-selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome-reacted spermatozoa. In conclusion, SLC through Androcoll (TM)-E does not adversely affect the capacitation-like status of stallion spermatozoa, although it did increase with time during cold storage.

Published in

Reproduction in Domestic Animals
2011, volume: 46, number: 1, pages: e74-e78
Publisher: Wiley-Blackwell

SLU Authors

• Bergqvist, Ann-Sofi

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Johannisson, Anders

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Dalin, Anne-Marie

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Rodriguez, Heriberto

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1111/j.1439-0531.2010.01637.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/30250