Bergqvist, Ann-Sofi
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Research article2011Peer reviewedOpen access
Bergqvist, A-S; Johannisson, A.; Backgren, L.; Dalin, A-M; Rodriguez-Martinez, H.; Morrell, J. M.
This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation-like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6 degrees C. Sperm capacitation-like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation-like status between colloid-selected and non-selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome-reacted spermatozoa. In conclusion, SLC through Androcoll (TM)-E does not adversely affect the capacitation-like status of stallion spermatozoa, although it did increase with time during cold storage.
Reproduction in Domestic Animals
2011, Volume: 46, number: 1, pages: e74-e78
Publisher: Wiley-Blackwell
Animal and Dairy Science
Veterinary Science
DOI: https://doi.org/10.1111/j.1439-0531.2010.01637.x
https://res.slu.se/id/publ/30250