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Research article2004Peer reviewedOpen access

Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial

Bjerketorp J, Rosander A, Nilsson M, Jacobsson K, Frykberg L


A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta(2)-glycoprotein I (beta(2)-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta(2)-GPI was also detected on all tested CVCs


Staphylococcus aureus; phage display; biomaterial; receptin; beta 2-glycoprotein I

Published in

Journal of Medical Microbiology
2004, Volume: 53, number: 10, pages: 945-951