Synthesis, physicochemical and biochemical studies of 1',2'-oxetane constrained adenosine and guanosine modified oligonucleotides, and their comparison with those of the corresponding cytidine and thymidine analogues

Abstract

We have earlier reported the synthesis and antisense properties of the conformationally constrained oxetane-C and -T containing oligonucleotides, which have shown effective down-regulation of the proto-oncogene c-myb mRNA in the K562 human leukemia cells. Here we report on the straightforward syntheses of the oxetane-A and oxetane-G nucleosides as well as their incorporations into antisense oligonucleotides (AONs), and compare their structural and antisense properties with those of the T and C modified AONs (including the thermostability and RNase H recruitment capability of the AON/RNA hybrid duplex by Michaelis-Menten kinetic analyses, their resistance in the human serum, as well as in the presence of exo and endonucleases)

Published in

Journal of the American Chemical Society
2004, Volume: 126, number: 37, pages: 11484-11499
Publisher: AMER CHEMICAL SOC

SLU Authors

• Gohil, Suresh

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

Publication identifier

DOI: https://doi.org/10.1021/ja0484171

Permanent link to this page (URI)

https://res.slu.se/id/publ/3137